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primary antibodies against jak2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc primary antibodies against jak2
    Primary Antibodies Against Jak2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+jak2/anti+jak2/pm40468940-54-0-12
    Average 90 stars, based on 1 article reviews
    primary antibodies against jak2 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Crosstalk between bone marrow-derived myofibroblasts and gastric cancer cells regulates cancer stemness and promotes tumorigenesis
    Article Snippet: The gels were transferred onto nitrocellulose membranes (Bio-Rad). .. The membranes were probed with specific primary antibodies against JAK2 (Cat. 3230S), p-JAK2 (Cat. 3771S), STAT3 (Cat. 9139S), p-STAT3-Y705 (Cat. 76315), Met (Cat. 8198), p-Met (Cat. 3077), Smad2/3 (Cat. 5678), p-Smad2 (Cat.8828), vimentin (Cat. 3879) (Cell signaling Technology), snail (Cat. 92547), E-cadherin (Cat. ab15148), TGF-β1 (Cat. ab66043 ), and IL-6 (Cat. ab6672), and β-actin (Cat. ab6276) (Abcam), incubated with secondary antibodies conjugated to IR fluorophore, Alexa Fluor 680 (Molecular Probes), or IRdye 800 (Rockland Immunochemicals). .. Antigen-antibody complexes were visualized by the ECL system (Amersham Biosciences, Piscataway, NJ) and scanned using the Odyssey Infrared Imaging System (Li-Cor Biosciences).

    Article Title: Biodegradable Nanoparticles Mediated Co-delivery of Erlotinib (ELTN) and Fedratinib (FDTN) Toward the Treatment of ELTN-Resistant Non-small Cell Lung Cancer (NSCLC) via Suppression of the JAK2/STAT3 Signaling Pathway
    Article Snippet: The SDS-polyacrylamide gels were transferred onto PVDF membranes (Millipore, Bedford, MA, United States). .. The membranes were incubated with specific primary antibodies against JAK2, phospho-JAK2 (p-JAK2), STAT3, p-STAT3, Survivin (Cell Signaling Technology, San Diego, CA, United States), and subsequently with HRP-conjugated secondary antibodies conjugated to Alexa Fluor 680, or IRdye 800 (Rockland Immunochemicals, Inc. Gilbertsville, PA, United States). .. The intensities of the protein bands were scanned using the Odyssey Infrared Imaging System (Li-Cor Biosciences, Lincoln, NE, United States).

    Article Title: MicroRNA-216a inhibits the metastasis of gastric cancer cells by targeting JAK2/STAT3-mediated EMT process
    Article Snippet: .. After being transferred to polyvinylidene fluoride membranes (Millipore, MA, USA), the proteins were incubated with primary antibodies against JAK2 (Cell Signaling Technology, Beverly, MA, USA), E-cadherin (Abcam, Cambridge, MA, USA), N-cadherin (Abcam), Vimentin (Abcam), p-STAT3 (Tyr705; Cell Signaling Technology), STAT3 (Cell Signaling Technology), Slug (Abcam), Snail (Abcam), Twist (Abcam) or GAPDH (Sigma, Santa Clara, CA, USA) at 4°C overnight, and further incubated in HRP-linked secondary antibodies (Santa Cruz Biotechnology, CA, USA) for 1 h at room temperature. .. Alpha Innotech (San Leandro, CA, USA) imaging software was used to quantify western blotting data.

    Control:

    Article Title: miR-204 suppresses non-small-cell lung carcinoma (NSCLC) invasion and migration by targeting JAK2.
    Article Snippet: .. The primary antibodies against JAK2 was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA), using a GADPH antibody (Cell Signaling Technology) as the internal control. .. Densitometric analysis was performed using the Labworks Image Acquisition and Analysis Software (UVP, Upland, CA, USA).

    other:

    Article Title: Scoparone Exerts Anti-Tumor Activity against DU145 Prostate Cancer Cells via Inhibition of STAT3 Activity
    Article Snippet: After blocking, the membrane was incubated with primary antibodies against Cyclin D 1 , phospho-pRb (ppRb), JAK2, phospho-JAK2 Tyr1007/1008 (pJAK2), Src, phospho-Src family Tyr416 (pSrc), STAT3, phospho-STAT3 Tyr705 (pSTAT3 Y705), phospho-STAT3 Ser727 (pSTAT3 S727), Survivin, c-Myc, SOCS3, and Bcl-2 (Cell Signaling, Beverly, MA, USA); Cyclin E (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and β-actin (Sigma-Aldrich).

    Article Title: A blockade of leukotriene-mediated Alox5 function provides a new strategy for the treatment of JAK2 V617F-induced polycythemia vera.
    Article Snippet: Unconjugated primary antibodies against AKT, JAK2, Caspase-3, and PARP were purchased from Cell Signaling Technology.

    Article Title: Prevention of ulcerative colitis by Huangqin decoction: reducing the intestinal epithelial cell apoptosis rate through the IFN-γ/JAK/ETS signalling pathway
    Article Snippet: The membranes were then incubated overnight with primary antibodies against IFN-γ (1:1000, Cell Signaling Technology, Boston, MA), IFITM3 (1:1000, Cell Signaling Technology, Boston, MA), JAK1 (1:1000, Cell Signaling Technology, Boston, MA), JAK2 (1:1000, Cell Signaling Technology, Boston, MA), ETS-1 (1:1000, Cell Signaling Technology, Boston, MA), STAT1 (1:1000, Cell Signaling Technology, Boston, MA) and Bcl2 (1:1000, Cell Signaling Technology, Boston, MA) at 4 °C with gentle shaking.



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    Image Search Results


    Effects of PBPs on modulating macrophage polarization and the expression of genes related to the JAK2/STAT3 pathway in tumors. ( A and B ) M2 markers (Arg-1, and CD206); ( C and D ) M1 markers (CD86, and iNOS); ( E and F ) JAK2 and STAT3 transcripts. Data are presented as mean ± SD (n = 3).

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: Pleione bulbocodioides Polysaccharides Reprogram Tumor-Associated Macrophages via JAK2/STAT3 Inhibition to Suppress Hepatocellular Carcinoma

    doi: 10.2147/JHC.S578276

    Figure Lengend Snippet: Effects of PBPs on modulating macrophage polarization and the expression of genes related to the JAK2/STAT3 pathway in tumors. ( A and B ) M2 markers (Arg-1, and CD206); ( C and D ) M1 markers (CD86, and iNOS); ( E and F ) JAK2 and STAT3 transcripts. Data are presented as mean ± SD (n = 3).

    Article Snippet: Primary antibodies against JAK2 (cat. no. AF6022), phospho-JAK2 (cat. no. AF3024), STAT3 (cat. no. AF6294), phospho-STAT3 (cat. no. AF3293), BCL-2 (cat. no. AF6139), and β-Actin (cat. no. AF7018) (Affinity Biosciences, Cincinnati, OH, USA).

    Techniques: Expressing

    Effects of PBPs on regulating protein expression related to JAK2/STAT3 signaling, apoptosis, and angiogenesis in tumors. ( A ) Representative Western blot images; ( B ) p-JAK2/JAK2; ( C ) p-STAT3/STAT3; ( D ) BCL-2; ( E ) BAX; ( F ) VEGF. Data are presented as mean ± SD (n = 3).

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: Pleione bulbocodioides Polysaccharides Reprogram Tumor-Associated Macrophages via JAK2/STAT3 Inhibition to Suppress Hepatocellular Carcinoma

    doi: 10.2147/JHC.S578276

    Figure Lengend Snippet: Effects of PBPs on regulating protein expression related to JAK2/STAT3 signaling, apoptosis, and angiogenesis in tumors. ( A ) Representative Western blot images; ( B ) p-JAK2/JAK2; ( C ) p-STAT3/STAT3; ( D ) BCL-2; ( E ) BAX; ( F ) VEGF. Data are presented as mean ± SD (n = 3).

    Article Snippet: Primary antibodies against JAK2 (cat. no. AF6022), phospho-JAK2 (cat. no. AF3024), STAT3 (cat. no. AF6294), phospho-STAT3 (cat. no. AF3293), BCL-2 (cat. no. AF6139), and β-Actin (cat. no. AF7018) (Affinity Biosciences, Cincinnati, OH, USA).

    Techniques: Expressing, Western Blot

    Pattern diagram of molecular docking: (a) Hesperetin-IL-6, (b) hesperetin-CYCS, (c) hesperetin-TGFB1, (d) hesperetin-IL1B, (e) hesperetin-SOCS3, and (f) hesperetin-CEPBA.

    Journal: Open Medicine

    Article Title: Mechanisms of hesperetin in treating metabolic dysfunction-associated steatosis liver disease via network pharmacology and in vitro experiments

    doi: 10.1515/med-2025-1215

    Figure Lengend Snippet: Pattern diagram of molecular docking: (a) Hesperetin-IL-6, (b) hesperetin-CYCS, (c) hesperetin-TGFB1, (d) hesperetin-IL1B, (e) hesperetin-SOCS3, and (f) hesperetin-CEPBA.

    Article Snippet: The primary antibodies against interleukin-6 (IL-6) (DF6087), p-JAK2 (AF3024), JAK2 (AF6022), p-STAT3 (AF3293), and STAT3 (AF6294) were purchased from Affinity Biosciences (OH, USA).

    Techniques:

    Effects of hesperetin on IL-6-STAT3-SOCS3 pathway in FFA-induced HepG2 cells. (a) IL-6 content in cell culture medium. (b) Relative protein levels of IL-6 in HepG2 cells. (c) Relative protein levels of p-STAT3/STAT3 and SOCS3 in HepG2 cells. (d) Glucose absorption in different groups. (e) Levels of IL-6 in HepG2 cells. (e) Relative protein levels of p-STAT3/STAT3 and SOCS3 in IL-6-induced HepG2 cells. The data represented as mean ± SD, n = 3. * P < 0.05, ** P < 0.01, compared to Ctrl; # P < 0.05, ## P < 0.01, compared to FFA.

    Journal: Open Medicine

    Article Title: Mechanisms of hesperetin in treating metabolic dysfunction-associated steatosis liver disease via network pharmacology and in vitro experiments

    doi: 10.1515/med-2025-1215

    Figure Lengend Snippet: Effects of hesperetin on IL-6-STAT3-SOCS3 pathway in FFA-induced HepG2 cells. (a) IL-6 content in cell culture medium. (b) Relative protein levels of IL-6 in HepG2 cells. (c) Relative protein levels of p-STAT3/STAT3 and SOCS3 in HepG2 cells. (d) Glucose absorption in different groups. (e) Levels of IL-6 in HepG2 cells. (e) Relative protein levels of p-STAT3/STAT3 and SOCS3 in IL-6-induced HepG2 cells. The data represented as mean ± SD, n = 3. * P < 0.05, ** P < 0.01, compared to Ctrl; # P < 0.05, ## P < 0.01, compared to FFA.

    Article Snippet: The primary antibodies against interleukin-6 (IL-6) (DF6087), p-JAK2 (AF3024), JAK2 (AF6022), p-STAT3 (AF3293), and STAT3 (AF6294) were purchased from Affinity Biosciences (OH, USA).

    Techniques: Cell Culture